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wnt β catenin pathway  (MedChemExpress)


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    MedChemExpress wnt β catenin pathway
    Meis1 regulated EMT <t>through</t> <t>the</t> <t>Wnt/β-catenin</t> pathway. ( A ) q-PCR analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids, ** P < .01. ( B ) Western blot analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids. ( C ) Western blot analysis of lentivirus-mediated Meis1 knockdown efficiency by shRNA in HaCaT cells. ( D ) Venn diagram showing overlapping differentially expressed genes (DEGs) between the TGF-β1-induced group and sh-Meis1-2 group (C1: the control group; C2: TGF-β1-induced group; C3: sh-Meis1-2 group). ( E ) Pathway enrichment analysis of overlapping DEGs between the TGF-β1-induced group and sh-Meis1-2 group. ( F-H ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 overexpression, * P < .05, ** P < .01. ( I ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 overexpression. ( J-L ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 knockdown, * P < .05, ** P < .01. ( M ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 knockdown. ( N ) Western blot analysis of β-catenin protein expression in cells with or without MSAB treatment. ( O ) Western blot analysis of α-SMA, Vimentin, E-cadherin, and N-cadherin protein expression in cells with or without MSAB treatment.
    Wnt β Catenin Pathway, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Meis1 Negatively Regulates Epithelial-Mesenchymal Transition via Wnt/β-catenin Pathway in Oral Submucous Fibrosis"

    Article Title: Meis1 Negatively Regulates Epithelial-Mesenchymal Transition via Wnt/β-catenin Pathway in Oral Submucous Fibrosis

    Journal: International Dental Journal

    doi: 10.1016/j.identj.2025.109323

    Meis1 regulated EMT through the Wnt/β-catenin pathway. ( A ) q-PCR analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids, ** P < .01. ( B ) Western blot analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids. ( C ) Western blot analysis of lentivirus-mediated Meis1 knockdown efficiency by shRNA in HaCaT cells. ( D ) Venn diagram showing overlapping differentially expressed genes (DEGs) between the TGF-β1-induced group and sh-Meis1-2 group (C1: the control group; C2: TGF-β1-induced group; C3: sh-Meis1-2 group). ( E ) Pathway enrichment analysis of overlapping DEGs between the TGF-β1-induced group and sh-Meis1-2 group. ( F-H ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 overexpression, * P < .05, ** P < .01. ( I ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 overexpression. ( J-L ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 knockdown, * P < .05, ** P < .01. ( M ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 knockdown. ( N ) Western blot analysis of β-catenin protein expression in cells with or without MSAB treatment. ( O ) Western blot analysis of α-SMA, Vimentin, E-cadherin, and N-cadherin protein expression in cells with or without MSAB treatment.
    Figure Legend Snippet: Meis1 regulated EMT through the Wnt/β-catenin pathway. ( A ) q-PCR analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids, ** P < .01. ( B ) Western blot analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids. ( C ) Western blot analysis of lentivirus-mediated Meis1 knockdown efficiency by shRNA in HaCaT cells. ( D ) Venn diagram showing overlapping differentially expressed genes (DEGs) between the TGF-β1-induced group and sh-Meis1-2 group (C1: the control group; C2: TGF-β1-induced group; C3: sh-Meis1-2 group). ( E ) Pathway enrichment analysis of overlapping DEGs between the TGF-β1-induced group and sh-Meis1-2 group. ( F-H ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 overexpression, * P < .05, ** P < .01. ( I ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 overexpression. ( J-L ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 knockdown, * P < .05, ** P < .01. ( M ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 knockdown. ( N ) Western blot analysis of β-catenin protein expression in cells with or without MSAB treatment. ( O ) Western blot analysis of α-SMA, Vimentin, E-cadherin, and N-cadherin protein expression in cells with or without MSAB treatment.

    Techniques Used: Knockdown, Transfection, Western Blot, shRNA, Control, Expressing, Over Expression



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    Meis1 regulated EMT <t>through</t> <t>the</t> <t>Wnt/β-catenin</t> pathway. ( A ) q-PCR analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids, ** P < .01. ( B ) Western blot analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids. ( C ) Western blot analysis of lentivirus-mediated Meis1 knockdown efficiency by shRNA in HaCaT cells. ( D ) Venn diagram showing overlapping differentially expressed genes (DEGs) between the TGF-β1-induced group and sh-Meis1-2 group (C1: the control group; C2: TGF-β1-induced group; C3: sh-Meis1-2 group). ( E ) Pathway enrichment analysis of overlapping DEGs between the TGF-β1-induced group and sh-Meis1-2 group. ( F-H ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 overexpression, * P < .05, ** P < .01. ( I ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 overexpression. ( J-L ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 knockdown, * P < .05, ** P < .01. ( M ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 knockdown. ( N ) Western blot analysis of β-catenin protein expression in cells with or without MSAB treatment. ( O ) Western blot analysis of α-SMA, Vimentin, E-cadherin, and N-cadherin protein expression in cells with or without MSAB treatment.
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    Image Search Results


    Meis1 regulated EMT through the Wnt/β-catenin pathway. ( A ) q-PCR analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids, ** P < .01. ( B ) Western blot analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids. ( C ) Western blot analysis of lentivirus-mediated Meis1 knockdown efficiency by shRNA in HaCaT cells. ( D ) Venn diagram showing overlapping differentially expressed genes (DEGs) between the TGF-β1-induced group and sh-Meis1-2 group (C1: the control group; C2: TGF-β1-induced group; C3: sh-Meis1-2 group). ( E ) Pathway enrichment analysis of overlapping DEGs between the TGF-β1-induced group and sh-Meis1-2 group. ( F-H ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 overexpression, * P < .05, ** P < .01. ( I ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 overexpression. ( J-L ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 knockdown, * P < .05, ** P < .01. ( M ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 knockdown. ( N ) Western blot analysis of β-catenin protein expression in cells with or without MSAB treatment. ( O ) Western blot analysis of α-SMA, Vimentin, E-cadherin, and N-cadherin protein expression in cells with or without MSAB treatment.

    Journal: International Dental Journal

    Article Title: Meis1 Negatively Regulates Epithelial-Mesenchymal Transition via Wnt/β-catenin Pathway in Oral Submucous Fibrosis

    doi: 10.1016/j.identj.2025.109323

    Figure Lengend Snippet: Meis1 regulated EMT through the Wnt/β-catenin pathway. ( A ) q-PCR analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids, ** P < .01. ( B ) Western blot analysis of Meis1 knockdown efficiency in HaCaT cells transfected with sh-Meis1 plasmids. ( C ) Western blot analysis of lentivirus-mediated Meis1 knockdown efficiency by shRNA in HaCaT cells. ( D ) Venn diagram showing overlapping differentially expressed genes (DEGs) between the TGF-β1-induced group and sh-Meis1-2 group (C1: the control group; C2: TGF-β1-induced group; C3: sh-Meis1-2 group). ( E ) Pathway enrichment analysis of overlapping DEGs between the TGF-β1-induced group and sh-Meis1-2 group. ( F-H ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 overexpression, * P < .05, ** P < .01. ( I ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 overexpression. ( J-L ) q-PCR analysis of β-catenin, cyclin D1, and c-Myc mRNA expression in cells with Meis1 knockdown, * P < .05, ** P < .01. ( M ) Western blot analysis of β-catenin, c-Jun, c-Myc, and cyclin D1 protein expression in cells with Meis1 knockdown. ( N ) Western blot analysis of β-catenin protein expression in cells with or without MSAB treatment. ( O ) Western blot analysis of α-SMA, Vimentin, E-cadherin, and N-cadherin protein expression in cells with or without MSAB treatment.

    Article Snippet: MSAB was used to treat HaCaT cells by blocking the Wnt/β-catenin pathway (MCE, HY-120697).

    Techniques: Knockdown, Transfection, Western Blot, shRNA, Control, Expressing, Over Expression